ObjectiveThe near-full-length genome (NFLG) sequence amplification and analysis were performed on the serum sample of a patient infected with HIV-1 by heterosexual contact in a border city of Guangxi, in order to understand the recombinant form and gene sequence characteristics. MethodsViral RNA from serum sample of research object was reverse transcribed, amplified, sequenced by reverse transcription-polymerase chain reaction(RT-PCR) and nested polymerase chain reaction (PCR), breakpoints of gene recombination were analyzed and recombinant form was confirmed, a subtype phylogenetic tree was constructed as well. ResultsSequencing results showed that the full length of the strain sequence was 8900 bp, which was a circulating recombinant form (CRF) 65-cpx recombinant subtype (B/C/CRF01-AE) strain keeping C as the skeleton with CRF01-AE and B subtype fragments inserted. The results of drug resistance analysis showed that there was a V179D resistant mutation in the gene sequence strain. The NFLG sequence of the recombinant strain was composed of CRF01-AE, B and C, with a sequence location of 790 to 9412 bp and 14 fragments, whose 13 breakpoints were 1170, 3803, 4015, 5655, 5830, 5993, 6378, 7586, 8349, 8506, 8568, 8830 and 8995. Fragments Ⅰ、Ⅴ、Ⅶ、Ⅹ and Ⅻ were subtypes of CRF01-AE. Fragments Ⅲ、Ⅵ、Ⅸ and ⅩⅢ were subtypes of B, and fragments Ⅱ、Ⅳ、Ⅷ、Ⅺ and ⅩⅣ were subtypes of C. ConclusionThe CRF65-cpx strain found in a Guangxi border city is composed of B, C and CRF01-AE subtype strains, and contained V179D mutation. Frontier of Guangxi ought to strengthen surveillance for the aforesaid subtype or novel-like virus strains, and take corresponding measures of preventing and controlling to avoid the spread and epidemic of non-domestic novel recombinant strains or domestic recombinant strains in the area.

无