内科

目的研究不同浓度米诺环素对大肠杆菌生物膜的影响。方法采用腹膜透析液－腹膜透析管系统建立大肠杆菌生物被膜模型，根据米诺环素浓度不同分为4组。通过银染法染色、结晶紫半定量测定、连续稀释法计算活菌计数，观察腹膜透析管表面生物膜的形成情况。结果培养6 h，1/2 最低抑菌浓度（MIC）米诺环素组、1/4MIC米诺环素组的载体表面银染快速鉴定均未发现形成生物膜，1/8MIC米诺环素组及对照组均形成早期生物膜。1/2MIC米诺环素组在培养12 h、24 h三个时间段内，生物膜内的活菌计数均少于对照组（P＜0.05）；生物膜结晶紫半定量均小于对照组（P＜0.05）；1/4MIC米诺环素组培养12 h生物膜结晶紫半定量均小于对照组及1/8MIC组。1/8MIC米诺环素组培养6 h、12 h、24 h，生物膜结晶紫半定量均低于对照组（P＜0.05）。结论米诺环素在体外可以抑制大肠杆菌生物膜的形成。

ObjectiveTo study the effects of minocycline with different concentrations on the biofilm of Escherichia coli. MethodsA model of Escherichia coli biofilm was established by using Liquor Dialysisintraperitoneus peritoneal dialysis catheter system. The concentration of minocycline was divided into 4 groups according to the concentration of minocycline. The biofilm formation on peritoneal dialysis tube was observed by silver staining, crystal violet quantitative analysis and continuous dilution method. Results1/2 minimum inhibitory concentration (MIC) minocycline group and 1/4MIC minocycline group were not identified as biofilms after 6h. 1/8MIC minocycline group and control group all formed early biofilms. In the three periods of culture 12h, 24h and 48h, the counts of viable bacteria in the 1/2MIC minocycline group were less than those in the control group (P＜0.05). The semi quantitative biological crystal violet was less than that of the control group (P＜0.05). The semi quantitation of biofilm crystal violet in 12h 1/4MIC minocycline group was less than that in control group and 1/8MIC group. In culture 6h, 12h and 24h, the semi quantitative 1/8MIC of minocycline group was lower than that of the control group (P＜0.05). ConclusionMinocycline can inhibit the formation of Escherichia coli biofilm in vitro.