ObjectiveTo investigate the changing of peripheral blood lymphocyte subgroup, LgG and complement( C3, C4) during different disease phases in patients with chronic hepatitis B virus (HBV) infection. MethodsA total of 80 patients with HBV infection were selected and divided into chronic hepatitis B group, cirrhosis group and acuteonchronic hepatitis B group, and 26 healthy individuals were enrolled as control group. Flow cytometry was used to detect the peripheral blood lymphocytes subsets and CD3-CD19+(B cell), serum immunoglobulin G and complements(C3 and C4) were detected by immune nephelometry. ResultsThe percentage of CD3+, CD4+ and CD8+ cells of peripheral blood decreased in the order of control group, chronic hepatitis B group, cirrhosis group and acuteonchronic hepatitis B group,whereas the percentage of B cell was increased in the above order; the percentage of CD3+, CD4+ and CD8+ cells of peripheral blood in the chronic hepatitis B group, cirrhosis group and acuteonchronic hepatitis B group were significant lower than those in control group, while the percentage of B cell was significant higher than that in control group (P＜0.05). The level of LgG increased in the order of control group, chronic hepatitis B group, cirrhosis group and acuteonchronic hepatitis B group,whereas the level of C3,C4 were decreased in the above order,there was significant difference among four groups (P＜0.05). ConclusionsThere is an imbalance in peripheral blood Tlymphocyte subsets and turbulence in cellular immunity in the patients with chronic hepatitis B, humoral immunity also changes obvious, the detection of peripheral blood lymphocyte subgroup, LgG and complement has applied value in the diagnosis and predict prognosis of chronic hepatitis B virus (HBV) infection.

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