内科

目的探讨TGF-β1对乙肝疫苗免疫应答的影响以及与HLA-DRB1*15基因的关系。方法选取完成重组乙型肝炎疫苗标准全程接种的健康大学生中抗-HBs S/N值＜10 mIU/ml者77名为无、弱应答组，选取抗-HBs S/N值＞10 mIU/ml 者101名为中、强应答组，应用PCR-SSP进行HLA-DRB1*15等位基因的检测，采用ELISA法检测血清中TGF-β1水平。结果①无、弱应答组的HLA-DRB1*15基因表达频率为14.29%（11/77），低于中、强应答组的28.71%（29/101）（P=0.022）；②无、弱应答组中TGF-β1的平均表达水平为（24.04±14.74）ng/ml，高于中、强应答组（17.80±14.42）ng/ml；差异有统计学意义（P=0.005）；③HLA-DRB1*15阳性组TGF-β1平均表达水平为（17.81±17.38）ng/ml，阴性组为（21.28±14.00）ng/ml，差异无统计学意义（P=0.194）。结论① HLA-DRB1*15基因可能是促进人群乙肝疫苗免疫应答的相关基因；②TGF-β1的表达水平可能影响机体乙肝疫苗的免疫效果；③ HLA-DRB1*15基因可能不是通过影响TGF-β1的表达水平来影响乙肝疫苗的免疫应答。

ObjectiveTo discuss the influence on the immune response of hepatitis B vaccine and dependencies with HLA-DRB1* 15 allele for TGF-β1 cytokine.Methods77 non-or-hypo-responders (anti-HBs S/N＜10mIU/ml) who had received the whole standard courses of immunization with recombinant hepatitis B vaccination and 101 medium-or-hyper-responders (anti-HBs S/N＞10mIU/ml) were drawn randomly for the subjects.HLA-DRB1 *15 was detected by PCR-SSP,and the level of TGF-β1 cytokines was examined by ELISA.Results①The allelic frequencies of HLA-DRB1*15 was lower in the non-or hypor-responders than that in the medium-or-hyper-responders (14.29% vs 28.71%,P=0.022); ② The expression level of TGF-β1 in the non-or-hypor-responders （24.04±14.74）ng/ml was higher than that of the medium-or-hyper- responders （17.80±14.42）ng/ml,the difference had statistical significance（P=0.005）. ③ No statistical significance differences was seen between the expression level of TGF-β1 in the HLA-DRB1*15 positive (17.81±17.38)ng/ml and the negative (21.28±14.00) ng/ml (P=0.194)).Conclusions① HLA-DRB1*15 might be the allele enhanced immune response to hepatitis B vaccination. ② The level of TGF-β1 might play a role in the immune response to hepatitis B vaccination. ③ HLA-DRB1*15 might take effect in the response to hepatitis B vaccination without the expression of TGF-β1.