Objective To investigate the effect of point injection of vitamin B12 on children with delayed language development. Methods A total of 81 children with delayed language development were selected and randomly divided into a control group (41 cases) or a study group (40 cases). The control group received language rehabilitation training, while the study group received language rehabilitation training combined with point injection of vitamin B12. The therapeutic effects and developmental quotient before and after treatment were compared between the two groups. Results After two courses of treatment, the total effective rates of comprehension ability and expressive ability in the study group were higher than those in the control group, and the developmental quotients in the social behavior, language ability, adaptive ability, gross motor, and fine motor functional areas of the Gesell Development Scale were all higher in the study group than in the control group (all P<0.05). Conclusion Point injection of vitamin B12 has a better effect in treating children with delayed language development and can improve their developmental quotients.