Objective To construct a nursing management plan based on the Clinical and Laboratory Standards Institute (CLSI) GP41-A7 Collection of Diagnostic Venous Blood Specimens and to evaluate its application effect. Methods (1) Construction of a nursing management plan: A research team was set up to form a first draft of the CLSI GP41-A7 Collection of Diagnostic Venous Blood Specimens-based nursing management plan by reviewing the literature and combining the practical experience and the actual situation of the region. The Delphi method was used in the development of the final draft, during which 2 rounds of expert consultation were conducted via email, WeChat, etc. in June and August 2022, respectively, and the items of the plan were revised according to the experts' opinions. (2) Evaluation of the application effect of the CLSI GP41-A7 Collection of Diagnostic Venous Blood Specimens-based nursing management plan: A total of 200 patients who were taken blood samples in the outpatient blood sampling room of Maternal and Child Health Care Hospital of Baise, Guangxi, from April 2022 to October 2023 were selected as the research subjects by convenience sampling method. According to the blood sampling time, patients undergoing blood sampling from April to December 2022 were divided into the control group, and those undergoing blood sampling from January to October 2023 were divided into the observation group, with 100 cases in each group. The control group received routine care in the outpatient blood sampling room, based on which the observation group was cared for according to the CLSI GP41-A7 Collection of Diagnostic Venous Blood Specimens-based nursing management plan. The incidence of adverse events in nursing, the incidence of blood specimen quality issues, and the satisfaction of patients with the nursing work in the blood sampling room were compared between the two groups. Results (1) The construction of a CLSI GP41-A7 Collection of Diagnostic Venous Blood Specimens-based nursing management plan: The plan consisted of 5 first-level items, including confirmation, evaluation, standard prevention, sampling, and specimen processing, and 14 second-level items. The response rates of the 2 rounds of questionnaires were 100%, with the expert authoritative coefficients being 0.841 and 0.859 and the Kendall coefficients of concordance being 0.351 and 0.374, respectively (all P<0.05); the mean importance score of all items ranged from 4.26 to 4.93, and the coefficient of variation of importance score ranged from 0.084 to 0.214; the consistency ratio of the first-and second-level items were less than 0.1. (2) The clinical application effect of the plan: The incidence of adverse events in nursing and the incidence of blood specimen quality issues in the observation group were lower than those in the control group, and patients' satisfaction with the nursing work in the blood sampling room was higher than that in the control group (all P<0.05). Conclusion The CLSI GP41-A7 Collection of Diagnostic Venous Blood Specimens-based nursing management plan has high scientificity and reliability, which can reduce the occurrence of adverse events in nursing and blood specimen quality issues in the outpatient blood sampling room, improve patients' satisfaction with the nursing work in blood sampling room, and enhance the nursing quality of outpatient blood sampling room.

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