Objective To analyze the 2022 disease spectrum and epidemiological characteristics of patients in the emergency treatment room (ER) of a emergency department of a tertiary general hospital in Nanning. Methods A retrospective analysis was performed using the medical records of 3,858 patients admitted to the ER of emergency department of a tertiary A general hospital in Nanning, Guangxi, from January 1 to December 31, 2022, including gender, age, time of consultation, main diagnosis, and ER length of stay. Results Among the 3,858 patients in the ER of the Department of Emergency, the male-to-female ratio was 1.73:1, and the age was 15-98 (58.16±19.00) years; the youngest-old group (> 60-75 years old) accounted for the largest proportion of patients, followed by the young group (≤44 years old) and the middle-aged group (> 44-60 years old). The incidence of acute and critical illness was the highest in spring, followed by those in autumn and winter, and that in summer was the lowest; most patients visited between 9:00 and 12:00, 15:00 and 17:00, and 18:00 and 22:00. In patients of the ER of emergency department, the top 5 disease spectrums were diseases of the nervous system (accounted for 26.0%, with 668 males and 334 females aged [60.36±16.55] years), diseases of the circulatory system (accounted for 25.8%, with 616 males and 379 females aged [61.90±16.96] years), diseases of the respiratory system (accounted for 14.8%, with 351 males and 221 females aged [66.50±17.79] years), diseases of the digestive system (accounted for 11.2%, with 327 males and 105 females aged [56.92±17.50] years), injury, poisoning or certain other consequences of external causes (accounted for 7.7%, with 175 males and 122 females aged [37.49±17.82] years). Among the patients with the aforementioned 5 types of diseases, the proportions of males were higher than those of females, and the age differences were statistically significant (all P<0.05). Among the 5 types of diseases, the proportion of cerebral infarction in diseases of the nervous system (52.4%) was the highest, followed by those of cerebral hemorrhage (27.6%) and epilepsy (5.0%); the proportion of heart failure in diseases of the circulatory system (27.4%) was the highest, followed by those of acute coronary syndrome (21.0%) and hypertensive emergency (19.2%); the proportion of pneumonia in diseases of the respiratory system (51.6%) was the highest, followed by those of chronic obstructive pulmonary disease (19.8%) and respiratory failure (8.0%); the proportion of gastrointestinal hemorrhage in diseases of the digestive system (44.2%) was the highest, followed by those of acute pancreatitis (14.6%) and acute cholecystitis (4.4%); the proportion of drug poisoning in injury, poisoning or some other consequences of external causes (38.7%) was the highest, followed by that of multiple injuries (38.0%). For patients in the ER of the emergency department, the median ER length of stay was 6.80 hours; 88.3% of the patients could be discharged from the ER within 24 hours, and 0.9% of the patients stayed in the ER for more than 72 hours; a total of 2,136 patients (55.4%) stayed in the ER for more than 6 hours, and the top 5 disease spectrums were diseases of the circulatory system, diseases of the nervous system, diseases of the respiratory system, diseases of the digestive system, and injury, poisoning or certain other consequences of external causes. Conclusion In 2022, the majority of patients in the ER of the emergency department of a tertiary A general hospital in Nanning, Guangxi, were cardiovascular and cerebrovascular diseases, respiratory and digestive system diseases, multiple injuries, and drug poisoning; most patients with acute and critical illness were males and aged >60-75 years old; the peak of ER visits in the emergency department located in spring, with a peak in the morning, afternoon, and evening, respectively, every day; most of the patients stayed in the ER for more than 6 hours.