ObjectiveTo explore the feasibility of one-dimensional gel electrophoresis (1-DE) in serum comparative proteomics research.MethodsUse a quantitative gel imaging software Quantity One to quantify each band in the electrophoresis map and then convert the graphical results into digital results.Electrophoresis maps of 32 patients and 32 health people′s extracted protein serum were studied to establish the feasibility of our study.ResultsThere were 10 bands with significant differences between the two groups (P＜0.05).We found 9 HCC markers with a matched molecular weight via document retrieval.ConclusionsThe method of the gel quantification software Quantity One is feasible for the study.We have proved that one-dimensional gel electrophoresis technique is feasible in early serum comparative proteomics research.

• ref